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Updated: Aug 17, 2026

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
D-L amino acid analysis using automated precolumn derivatization with 1-fluoro-2,4-dinitrophenyl-5-alanine amide
A Scaloni1, M Simmaco, F Bossa
1Dipartimento di Scienze Biochimiche "A. Rossi Fanelli", Centro di Biologia Molecolare del Consiglio Nazionale delle Ricerche, Università "La Sapienza", Piazzale Aldo Moro 5, I-00185, Roma, Italy.
Abstract:
An automated procedure for the precolumn derivatization of enantiomeric amino acid mixtures with 1-fluoro-2,4-dinitrophenyl-5-alanine amide and a liquid chromatographic method for the separation of the derivatives with UV detection are reported. The system described allows to perform routine analyses using microbore columns with a sensitivity at the picomol level. Improvements for the use of this reagent in the protocol of a subtractive Edman degradation procedure of peptides to determine the sequence position of amino acid residues with concomitant identification of their chirality are also described.
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