Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Growth Measurement: Indirect Methods01:27

Microbial Growth Measurement: Indirect Methods

2.3K
Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...
2.3K
Microbial Growth Measurement: Direct Methods01:23

Microbial Growth Measurement: Direct Methods

2.9K
Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...
2.9K
Methods to Assess Microbial Populations01:30

Methods to Assess Microbial Populations

103
Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a...
103
Bioreactor Controls-I01:28

Bioreactor Controls-I

104
Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly...
104

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of nutrient enrichment on surface microbial community gene expression in the oligotrophic North Pacific Subtropical Gyre.

The ISME journal·2018
Same author

Enigmatic relationship between chlorophyll a concentrations and photosynthetic rates at Station ALOHA.

Heliyon·2016
Same author

The grounding of the Bahia Paraiso: Microbial ecology of the 1989 Antarctic oil spill.

Microbial ecology·2013
Same author

Clinical predictors of risk of hypoglycaemia during addition and titration of insulin glargine for type 2 diabetes mellitus.

Diabetes, obesity & metabolism·2013
Same author

A novel method for the measurement of dissolved adenosine and guanosine triphosphate in aquatic habitats: applications to marine microbial ecology.

Journal of microbiological methods·2001
Same author

Unicellular cyanobacteria fix N2 in the subtropical North Pacific Ocean.

Nature·2001

Related Experiment Video

Updated: May 6, 2026

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation
12:04

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Published on: December 6, 2013

14.7K

Accurate estimation of microbial loop processes and rates.

D M Karl1

  • 1Department of Oceanography, School of Ocean and Earth Science and Technology, University of Hawaii, 96822, Honolulu, Hawaii, USA.

Microbial Ecology
|November 5, 2013
PubMed
Summary
This summary is machine-generated.

Conceptual models for aquatic microbial food webs offer a unifying hypothesis for field studies. Rigorous testing depends on precise methods, necessitating advancements in current techniques for accurate in situ microbial process resolution.

More Related Videos

Enhanced Reproducibility and Precision of High-Throughput Quantification of Bacterial Growth Data Using a Microplate Reader
09:15

Enhanced Reproducibility and Precision of High-Throughput Quantification of Bacterial Growth Data Using a Microplate Reader

Published on: July 27, 2022

2.4K
Precise, High-throughput Analysis of Bacterial Growth
09:00

Precise, High-throughput Analysis of Bacterial Growth

Published on: September 19, 2017

23.8K

Related Experiment Videos

Last Updated: May 6, 2026

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation
12:04

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Published on: December 6, 2013

14.7K
Enhanced Reproducibility and Precision of High-Throughput Quantification of Bacterial Growth Data Using a Microplate Reader
09:15

Enhanced Reproducibility and Precision of High-Throughput Quantification of Bacterial Growth Data Using a Microplate Reader

Published on: July 27, 2022

2.4K
Precise, High-throughput Analysis of Bacterial Growth
09:00

Precise, High-throughput Analysis of Bacterial Growth

Published on: September 19, 2017

23.8K

Area of Science:

  • Ecology
  • Microbiology
  • Limnology

Background:

  • Conceptual models of microbial food webs in aquatic ecosystems are crucial for hypothesis generation.
  • The advancement of ecological understanding is contingent upon the ability to rigorously test these hypotheses in the field.

Purpose of the Study:

  • To present a unifying hypothesis derived from conceptual models of aquatic microbial food webs.
  • To highlight the critical role of methodological precision and accuracy in validating these ecological hypotheses.

Main Methods:

  • Review of existing methodologies for assessing in situ microbial rates and processes.
  • Analysis of the limitations of current techniques in providing unambiguous resolution.
  • Identification of the need for improved or novel methodologies.

Main Results:

  • Conceptual models provide a framework for designing and conducting aquatic microbial ecology field studies.
  • Significant progress in methodological development has been achieved over the past two decades.
  • Current methods still present challenges for the unambiguous resolution of in situ microbial rates and processes.

Conclusions:

  • Conceptual models offer a valuable unifying hypothesis for aquatic microbial food web research.
  • The validation of these hypotheses is fundamentally limited by the precision and accuracy of available methods.
  • Future research must focus on developing improved or novel methodologies to overcome current limitations and achieve unambiguous resolution of microbial dynamics.