Related Experiment Video
Updated: May 6, 2026
Non-Invasive Endotracheal Administration of Lipopolysaccharide to Induce Acute Lung Injury in Rodents
Published on: December 5, 2025
Intervention effect and dose-dependent response of tanreqing injection on airway inflammation in
Shoujin Dong1, Yunqing Zhong, Kun Yang
1Pneumology Group, Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, West China School of Medicine, Sichuan University, Chengdu 610041, China. wuming-shoujin@sina.com
Objective:
To assess the effect of Tanreqing injection on airway inflammation in rats.
Methods:
A rat model of airway inflammation was generated with lipopolysaccharide (LPS). Tanreqing injection was given by intratracheal instillation, and bronchoalveolar lavage fluid (BALF) from the right lung was collected. BALF total cell and neutrophil counts were then determined. In addition, BALF levels of inflammatory cytokines interleukin-13, cytokine-induced neutrophil chemoat-tractant-1, and tumor necrosis factor-alpha were measured using enzyme linked immunosorbent assay. The middle lobe of the right lung was stained with hematoxylin-eosin and histological changes examined.
Results:
LPS increased airway inflammation, decreased BALF inflammatory cell count, inflammatory cytokine levels, and suppressed leukocyte influx of the lung. The LPS-induced airway inflammation peaked at 24 h, decreased beginning at 48 h, and had decreased markedly by 96 h.
Conclusion:
Tanreqing injection contains anti-inflammatory properties, and inhibits airway inflammation in a dose-dependent manner.
