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Loss of 2 um DNA from Saccharomyces cerevisiae transformed with the chimaeric plasmid pJDB219
M J Dobson1, A B Futcher, B S Cox
1Botany School, Sóuth Parks Road, 0X1 3RA, Oxford, England.
Current Genetics
|November 6, 2013
Abstract:
Two plasmids containing Saccharomyces cerevisiae 2 µm DNA sequences and the S. cerevisiae LEU2 gene have been found to display incompatibility with 2 µm DNA; in the presence of the LEU2 plasmids, 2 µm DNA can be lost. The LEU2 plasmids can be lost spontaneously after (and before) 2 µm DNA loss has occurred, so that strains completely lacking 2 µm DNA sequences can be obtained routinely.

