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Updated: May 6, 2026

In Vitro Reassociation Assay to Measure the Formation of 80S Ribosomal Particles Using Salt-washed Ribosomal Subunits
Published on: December 16, 2025
4OS ribosomal protein from a Saccharomyces cerevisiae antisuppressor mutant exhibiting a unique 2D gel pattern
1Department of Biological Sciences, University of Illinois at Chicago Circle, Box 4348, 60680, Chicago, Illinois, USA.
Abstract:
The yeast antisuppressor mutation, asu9-1 (Liebman and Cavenagh 1980) was found to cause an alteration in the 40S ribosomal subunit. Two-dimensional polyacrylamide gel electrophoresis patterns of the 40S ribosomal proteins from four different strains bearing the asu9-1 mutation all contained the same extra protein spot which was completely absent in five strains which did not carry the asu9 mutation.
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