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Truncated serine/arginine-rich splicing factor 3 accelerates cell growth through up-regulating c-Jun expression
Shizuka Kano1, Kensei Nishida, Chihiro Nishiyama
1Department of Stress Science, Institute of Health Biosciences, the University of Tokushima Graduate School.
The Journal of Medical Investigation : JMI
|November 6, 2013
Summary
Stressful conditions induce a truncated SRSF3 protein (SRSF3-TR) that accelerates cell growth by promoting G1 phase progression via enhanced c-Jun activity.
Area of Science:
- Molecular Biology
- Cell Biology
- Gene Regulation
Background:
- Serine/arginine-rich splicing factor 3 (SRSF3) is crucial for gene expression.
- SRSF3 produces a functional protein and a nonsense-mediated decay target (SRSF3-PTC).
- SRSF3-PTC can be translated into SRSF3-TR under stress.
Purpose of the Study:
- Investigate the functions of the stress-inducible SRSF3-TR.
- Determine the role of SRSF3-TR in cell cycle regulation and growth.
Main Methods:
- Established Flp-In-293 cells stably expressing SRSF3-TR.
- Analyzed mRNA and protein levels of cell cycle regulators.
- Assessed JUN promoter activity and transcription factor levels.
Main Results:
- SRSF3-TR expression accelerated cell growth.
- Increased levels of G1/S phase regulators (cyclin D1, D3, CDC25A, E2F1) were observed.
- JUN promoter activity, c-Jun, and Sp-1 levels were elevated.
Conclusions:
- Stress-inducible SRSF3-TR promotes cell growth.
- SRSF3-TR facilitates G1 progression through c-Jun activation.
- This mechanism may enhance cell proliferation under stress.
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