Plants from haploid tissue culture of Lavix decidua

Haploid embryogenic tissue was initiated on 1/2 LM medium supplemented with 500 mg/l glutamine, 1000 mg/l casein hydrolysate, 100 mg/l inositol, 30000 mg/l sucrose, and 0.1 mg/l 2,4-D. The embryoids matured to produce plantlets. One plant from one of the two lines survived. The chromosome complement of tissue cultures, of the needle bases from the source plant, and of the plant produced in vitro were established by squashes. DNA content was assessed by DNA microdensiometry. In vitro tissues were haploid (n = 12). The plant produced was mixoploid, with a predominance of diploid cells (2n = 24).