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Mapping oligogenic resistance to powdery mildew in mungbean with RFLPs
N D Young1, D Danesh, D Menancio-Hautea
1Department of Plant Pathology, University of Minnesota, 55108, St. Paul, MN, USA.
Abstract:
We have used restriction fragment length polymorphisms (RFLPs) to map genes in mungbean (Vigna radiata) that confer partial resistance to the powdery mildew fungus, Erysiphe polygoni. DNA genotypes for 145 RFLP loci spanning 1570 centimorgans of the mungbean genome were assayed in a population of 58 F2 plants. This population was derived from a cross between a moderately powdery mildew resistant ("VC3980A") and a susceptible ("TC1966") mungbean parent. F3 lines derived from the F2 plants were assayed in the field for powdery mildew response and the results were compared to the RFLP genotype data, thereby identifying loci associated with powdery mildew response. A total of three genomic regions were found to have an effect on powdery mildew response, together explaining 58% of the total variation. At 65 days after planting, two genomic regions were significantly associated with powdery mildew resistance. For both loci, the allele from "VC3890A" was associated with increased resistance. At 85 days, a third genomic region was also associated with powdery mildew response. For this locus, the allele from the susceptible parent ("TC1966") was the one associated with higher levels of powdery mildew resistance. These results indicate that putative partial resistance loci for powdery mildew in mungbean can be identified with DNA markers, even in a population of modest size analyzed at a single location in a single year.
Insights
Researchers mapped genes for powdery mildew resistance in mungbean (Vigna radiata) using restriction fragment length polymorphisms (RFLPs). DNA markers identified three genomic regions influencing resistance, aiding future breeding efforts.
Area of Science:
- Plant genetics and breeding
- Molecular markers for crop improvement
- Plant pathology and disease resistance
Background:
- Powdery mildew (Erysiphe polygoni) significantly impacts mungbean (Vigna radiata) yield and quality.
- Partial resistance genes are crucial for sustainable crop protection strategies.
- Genetic mapping provides insights into the inheritance of disease resistance traits.
Purpose of the Study:
- To identify and map genomic regions associated with partial resistance to powdery mildew in mungbean.
- To utilize DNA markers for efficient selection of resistant genotypes.
- To understand the genetic basis of mungbean's response to Erysiphe polygoni.
Main Methods:
- Restriction Fragment Length Polymorphisms (RFLPs) were employed to genotype 145 loci across the mungbean genome.
- A population of 58 F2 plants, derived from a cross between resistant and susceptible parents, was analyzed.
- Field assays of F3 lines evaluated powdery mildew response, correlated with RFLP data.
Main Results:
- Three genomic regions were identified, collectively explaining 58% of the variation in powdery mildew response.
- Two regions showed significant association with resistance at 65 days post-planting, with alleles from the resistant parent conferring increased resistance.
- A third region associated with resistance at 85 days post-planting involved an allele from the susceptible parent.
Conclusions:
- DNA markers, including RFLPs, can effectively identify partial resistance loci in mungbean, even with moderate population sizes.
- The identified genomic regions offer potential targets for marker-assisted selection in mungbean breeding programs.
- Understanding the genetic architecture of resistance, including potentially complex interactions, is vital for developing durable disease management strategies.
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