Somaclonal variation in watercress for resistance to crook root disease
D L Arnold1, A Flegmann, J M Clarkson
1School of Biological Sciences, University of Bath, BA2 7AY, Claverton Down, Bath Avon, UK.
Abstract:
Watercress (Rorippa nasturtium-aquaticum) shoots were regenerated from callus initiated from petioles on a range of media. The best coefficient of regeneration was obtained when callus was induced on a medium containing 0.25 mgl(-1) thidiazuron and 0.05 mgl{si-1} 2,4-D, followed by regeneration on 0.25 mgl(-1) thidiazuron. Plants were then screened for resistance to crook root disease of watercress in a laboratory screening test which revealed significant somaclonal variation between the controls and some of the somaclones.
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