Related Experiment Video

Updated: May 6, 2026

Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis
09:44

Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis

Published on: June 13, 2021

2.3K

Fungal microsomes in a biotransformation perspective: protein nature of membrane-associated reactions

Kateřina Svobodová1, Hana Mikesková, Denisa Petráčková

  • 1Laboratory of Environmental Biotechnology, Institute of Microbiology ASCR, v.v.i., Videnska 1083, 14220, Prague, Czech Republic, ksvobod@biomed.cas.cz.

Applied Microbiology and Biotechnology
|November 6, 2013
PubMed

Insights

Fungal microsomes contain enzymes like CYP450s crucial for metabolizing pollutants. This study reviews identified microsomal proteins, highlighting the need for further research into fungal biotransformation processes.

Area of Science:

  • Biochemistry
  • Microbiology
  • Proteomics

Background:

  • Microsomal fractions in fungal cells are rich in enzymes, particularly Cytochrome P450s (CYP450s).
  • These enzymes are vital for metabolizing diverse xenobiotic compounds, including polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), dioxins, and endocrine disruptors.
  • Understanding the regulation and function of these membrane-associated enzymes is crucial for biotechnological applications.

Purpose of the Study:

  • To review proteins identified in fungal microsomes using advanced proteomic techniques.
  • To investigate the role of microsomal enzymes in fungal biotransformation and biodegradation reactions.
  • To deepen the understanding of fungal intracellular processes related to xenobiotic metabolism.

Main Methods:

  • Shotgun proteomic approach was employed to identify proteins.
  • Analysis of fungal microsomes under various culture conditions.
  • Utilized advanced proteomic and post-genomic techniques.

Main Results:

  • Identified a significant number of microsomal proteins involved in fungal biotransformation.
  • Provided insights into the protein composition of fungal microsomes.
  • Highlighted the potential of fungal enzymes in metabolizing a wide array of xenobiotics.

Conclusions:

  • Fungal microsomes harbor enzymes critical for xenobiotic metabolism and biodegradation.
  • Further research is essential to fully elucidate the function and regulation of these microsomal enzymes.
  • Advanced proteomic methods are valuable tools for studying fungal intracellular processes.

More Related Videos

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy10:49

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

Published on: March 5, 2017

12.9K
A Whole Cell Bioreporter Approach to Assess Transport and Bioavailability of Organic Contaminants in Water Unsaturated Systems13:16

A Whole Cell Bioreporter Approach to Assess Transport and Bioavailability of Organic Contaminants in Water Unsaturated Systems

Published on: December 24, 2014

10.0K

Related Concept Videos

Drug Biotransformation: Overview01:16

Drug Biotransformation: Overview

Pharmaceutical substances known as xenobiotics are predominantly lipophilic and nonionized. This enables them to permeate lipid bilayers, such as cell membranes, and interact with intracellular target receptors. Lipophilic drugs have an advantage in crossing biological barriers and reaching their intended sites of action. However, lipophilic drugs often have a restricted capacity for renal expulsion or elimination from the body. When these drugs enter the kidneys and undergo glomerular...
3.7K
Drug Biotransformation: Overview01:28

Drug Biotransformation: Overview

Biotransformation, also known as drug metabolism, is a vital physiological process that chemically alters drugs, facilitating their elimination from the body and terminating their action. This process involves two main phases: phase I and phase II reactions. Phase I reactions, including oxidation, reduction, and hydrolysis, introduce or unmask polar functional groups on the drug molecule, thereby increasing its water solubility. By enhancing water solubility, the drug becomes more hydrophilic...
6.8K
Drug Metabolism: Phase II Reactions01:14

Drug Metabolism: Phase II Reactions

Phase II reactions are essential for the detoxification and elimination of drugs from the body. These reactions involve the conjugation of parent drugs or their phase I metabolites with endogenous molecules, resulting in more hydrophilic drug conjugates. The primary conjugation reactions in this phase are sulfation and glucuronidation. Both sulfation and glucuronidation typically produce biologically inactive metabolites. However, in some cases involving prodrugs, active metabolites may be...
4.7K
Antifungal Agents01:15

Antifungal Agents

Amphotericin B is a broad-spectrum antifungal agent that exploits structural differences between fungal and mammalian cell membranes. Its amphipathic structure—featuring a hydrophobic polyene-lactone ring and a hydrophilic region containing mycosamine and carboxylic acid groups—enables selective binding to ergosterol, a sterol predominantly found in fungal plasma membranes. This selective interaction underlies the drug’s antifungal activity, although weak binding to...
132
Phase II Reactions: Sulfation and Conjugation with α-Amino Acids01:19

Phase II Reactions: Sulfation and Conjugation with α-Amino Acids

Sulfation and α-amino acid conjugation are two critical biotransformation reactions in drug metabolism. Sulfation, a phase II biotransformation reaction, involves adding a polar sulfate group to a drug, enhancing its water solubility and promoting excretion. This process can either co-occur with or occur independently of glucuronidation. Nonmicrosomal sulfotransferase enzymes catalyze the process. The reaction involves 3'-phosphoadenosine-5'-phosphosulfate or PAPS coenzyme...
1.4K
Factors Affecting Drug Biotransformation: Biological01:19

Factors Affecting Drug Biotransformation: Biological

Biological factors significantly impact drug metabolism, influencing drug clearance, efficacy, and potential toxicity.
Species differences: Variations in enzyme systems across species can cause disparities in drug metabolism. For instance, humans may metabolize certain drugs faster than rodents, altering therapeutic effects.
Strain differences: Genetic variations within a species can result in differing enzyme activity, impacting drug response and toxicity. For example, some mouse strains may...
925