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Influence of phosphate ion on the fluorescence of 3-fluorotyrosine
S I Vdovenko1, M T Kolycheva, I I Gerus
1Institute of Bioorganic and Oil Chemistry, Academy of Sciences of Ukraine, Murmanskaja street, 1 Kiev-94, 253660, Ukraine.
Abstract:
3-Fluorotyrosine fluorescence is quenched effectively by phosphate ions not only by a dynamic but also by a static mechanism owing to H-bond complex formation in ground state. 3-Fluorotyrosine pKa values both in the ground and first excited state (8.3 and 4, respectively) are appreciably lower than those of tyrosine, thus promoting 3-fluorotyrosinate ion formation in the excited state. Additional emission owing to 3-fluorotyrosinate ion (near 350 nm) may be taken erroneously for tryptophan fluorescence.
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