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Isolation of Mouse Endometrial Epithelial and Stromal Cells for In Vitro Decidualization
Published on: March 2, 2017
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Differential expression and regulation of Tdo2 during mouse decidualization
Dang-Dang Li1, Ying-Jie Gao, Xue-Chao Tian
1College of Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China.
The Journal of Endocrinology
|November 6, 2013
Summary
Tryptophan 2,3-dioxygenase (Tdo2) is crucial for mouse decidualization, with its expression regulated by hormones and cAMP. Tdo2 influences cell proliferation and gene expression during pregnancy.
Area of Science:
- Reproductive biology
- Enzymology
- Molecular genetics
Background:
- Tryptophan 2,3-dioxygenase (Tdo2) catalyzes the initial step in tryptophan metabolism to kynurenine.
- Decidualization is a critical process for successful pregnancy, involving uterine tissue transformation.
Purpose of the Study:
- To investigate the expression and regulation of Tdo2 during mouse decidualization.
- To understand Tdo2's role in uterine stromal and decidual cells.
Main Methods:
- Real-time PCR to quantify Tdo2 mRNA expression in mouse uteri.
- In vivo and in vitro models of artificial decidualization.
- Hormonal and cAMP treatments on ovariectomized mice and uterine cells.
- Gene expression analysis following Tdo2 manipulation (overexpression and inhibition).
Main Results:
- Tdo2 mRNA expression is significantly upregulated in the decidua during days 6-8 of pregnancy.
- Estrogen, progesterone, and cAMP induce Tdo2 expression in uterine cells.
- Tdo2 regulates cell proliferation and stimulates decidual marker Dtprp expression.
- Tdo2 influences the expression of Ahr, Cox2, and Vegf genes, while its inhibition affects Cox2 and Vegf.
Conclusions:
- Tdo2 plays a significant role in mouse decidualization.
- Tdo2 expression is regulated by estrogen, progesterone, and cAMP.
- Tdo2 impacts key cellular processes and gene expression during decidualization.
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