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MiR-99a may serve as a potential oncogene in pediatric myeloid leukemia

Lidan Zhang, Xiaojuan Li, Zhiyong Ke

  • 1Department of Pediatric, The First Affiliated Hospital of Sun Yat-Sen University, Zhongshan Er Lu, Guangzhou 510080, China. huazhang420@gmail.com.

Cancer Cell International
|November 7, 2013
PubMed
Abstract

Insights

MicroRNA-99a (miR-99a) is highly expressed in pediatric myeloid leukemias, promoting cancer cell growth and inhibiting apoptosis. This suggests miR-99a is a potential therapeutic target for acute myeloid leukemia (AML) and chronic myeloid leukemia (CML).

Area of Science:

  • Hematology
  • Molecular Biology
  • Oncology

Background:

  • Leukemia is a prevalent childhood malignancy.
  • Previous studies identified miR-99a dysregulation in pediatric acute myeloid leukemia (AML).
  • The specific role of miR-99a in pediatric AML and chronic myeloid leukemia (CML) requires further investigation.

Purpose of the Study:

  • To investigate the expression and function of miR-99a in pediatric myeloid leukemias.
  • To explore the potential oncogenic role of miR-99a and its molecular targets.

Main Methods:

  • Quantitative reverse transcription polymerase chain reaction (qRT-PCR) for miR-99a expression analysis.
  • Cell proliferation (MTT assay) and apoptosis (Annexin V/propidium iodide staining) assays.
  • Dual-luciferase reporter assays and western blot analysis to identify miR-99a targets.

Main Results:

  • miR-99a was significantly upregulated in pediatric AML and CML, and downregulated during remission.
  • miR-99a promoted proliferation and inhibited apoptosis in K562 and HL60 leukemia cell lines.
  • miR-99a potentially targets tumor suppressor genes CTDSPL and TRIB2.

Conclusions:

  • miR-99a exhibits oncogenic activity in pediatric myeloid leukemias by regulating CTDSPL and TRIB2.
  • Pediatric AML and CML may share common disease pathways involving miR-99a.
  • miR-99a represents a potential common therapeutic target for myeloid leukemias.

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