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Patch Clamp

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Updated: May 6, 2026

A Computer-assisted Multi-electrode Patch-clamp System

A Computer-assisted Multi-electrode Patch-clamp System

Published on: October 18, 2013

A computer-assisted multi-electrode patch-clamp system.

Rodrigo Perin¹, Henry Markram

¹Laboratory of Neural Microcircuitry - Brain Mind Institute, Ecole Polytechnique Federale de Lausanne.

Journal of Visualized Experiments : Jove

|November 7, 2013

Summary

This summary is machine-generated.

This study introduces a computer-assisted system to simplify multi-electrode patch-clamp recordings. The new approach enhances experimental efficiency and success rates for neuronal electrical activity measurements.

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Last Updated: May 6, 2026

A Computer-assisted Multi-electrode Patch-clamp System

A Computer-assisted Multi-electrode Patch-clamp System

Published on: October 18, 2013

One-channel Cell-attached Patch-clamp Recording

One-channel Cell-attached Patch-clamp Recording

Published on: June 9, 2014

Application of Electrophysiology Measurement to Study the Activity of Electro-Neutral Transporters

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Area of Science:

Neuroscience
Biophysics
Electrophysiology

Background:

The patch-clamp technique is crucial for recording neuronal electrical activity.
Achieving stable patch-clamp recordings is complex and time-consuming.
Large-scale recordings require improved experimental efficiency and reliability.

Purpose of the Study:

To develop a computer-assisted system for multi-electrode patch-clamp experiments.
To simplify experimental steps and reduce human error.
To enhance the efficiency and success rate of neuronal recordings.

Main Methods:

Developed a computer-assisted system integrating controls via a wireless gamepad.
Centralized multi-electrode patch-clamp experiment controls into a single interface.
Incorporated visual feedback and guidance cues on the computer screen.

Main Results:

Reduced the time required for achieving recording configurations.
Substantially increased the success rate of recording large numbers of neurons simultaneously.
Enabled more reliable and efficient large-scale neuronal recordings.

Conclusions:

A simplified, computer-assisted approach enhances patch-clamp experiment efficiency.
Integrating experimenter experience with visual feedback improves recording success.
The developed system facilitates large-scale neuronal electrical activity measurements.