Related Experiment Video
Updated: May 6, 2026

Efficient Regeneration-based Agrobacterium-Mediated Transformation of an Asexual Amphibious Brassicaceae Species, Rorippa aquatica
Published on: January 16, 2026
Genetic factors influencing regeneration ability in rye (Secale cereale L.). I. Immature inflorescences
M Rakoczy-Trojanowska1, S Malepszy
1Institute of Horticultural Plant Genetics and Breeding, Warsaw Agricultural University, 02-766, 166, Warszawa, Nowoursynowska, Poland.
Abstract:
Immature inflorescences of ten rye inbred lines (inbred degree S10 and S11) were cultured on solidified MS medium supplemented with 3.0 mg/dm(3) of 2,4-D. According to their capability for callus production explants were classified into two groups : responsive (giving weak or intensive callus production) and non responsive (lack of callus formation). After transferring responsive material into hormone-free medium the regeneration of roots or shoots from the intensive growing callus was observed. Consistent differences between lines in the portion of explants with a certain response were found. They were divided into five groups reacting in the same way. Lines with different in-vitro response were crossed in an incomplete diallel. F1, F2 and F3 generations were analyzed and the following conclusions drawn: the ability for plant regeneration from immature inflorescences in rye is determined by numerous loci, has a recessive character, and both callus production and regeneration suppression may be controlled by complementary genes.
More Related Videos
08:52Author Spotlight: Optimizing Hairy Root-Based Transformation Protocols for Enhanced Efficiency in Brassicaceae
Published on: December 22, 2023
09:43Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
Published on: January 3, 2025
Related Concept Videos
Overview of Regeneration and Repair
Regeneration
All animals have varying degrees of...
Gene Regulation During Sporulation
Asexual Reproduction
Primary and Secondary Growth in Roots and Shoots
Morphogenesis
Whole Body Regeneration