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Sweet cherry cultivar identification by leaf isozyme polymorphism.

A R Granger¹, G R Clarke, J F Jackson

¹Department of Horticulture, Viticulture and Oenology, Waite Agricultural Research Institute, University of Adelaide, 5064, Glen Osmond, South Australia, Australia.

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik

|November 7, 2013

Summary

This summary is machine-generated.

Isozyme analysis reveals unique genetic profiles for sweet cherry cultivars. This method distinguishes between cultivars with similar appearances and identifies irradiated mutants, aiding in accurate cherry variety identification.

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Area of Science:

Horticulture and Plant Breeding
Biochemistry and Molecular Biology

Background:

Accurate identification of cherry cultivars is crucial for breeding programs and commercial trade.
Morphological characteristics can be insufficient for distinguishing between closely related or somaclonal variant cherry cultivars.

Purpose of the Study:

To investigate the utility of isozyme polymorphism for differentiating sweet cherry (Prunus avium) cultivars.
To establish unique genotype profiles for a wide range of sweet cherry varieties using multiple isozyme markers.

Main Methods:

Leaf extracts from 78 sweet cherry cultivars were analyzed for isozyme polymorphism.
Ten isozymes (6PGD, G6PD, GPI, IDH, PGM, FDP, SKDH, PER, MDH, GOT) were examined, each varying at a single locus.
Genotype profiles were generated to assess cultivar distinctiveness.

Main Results:

Isozyme analysis generated 70 unique genotype profiles from 78 sweet cherry cultivars.
Polymorphism was newly identified in eight isozymes (6PGD, G6PD, GPI, IDH, PGM, FDP, SKDH, PER).
Isozyme genotypes successfully differentiated cultivars with identical morphological traits and distinguished irradiated mutants from parent material.

Conclusions:

Isozyme polymorphism provides a reliable method for the precise identification and differentiation of sweet cherry cultivars.
This molecular approach enhances cultivar classification beyond traditional morphological assessments.
Isozyme profiling is valuable for cultivar registration, genetic resource management, and distinguishing between mutant and original plant material.