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Related Experiment Video

Updated: May 6, 2026

Floral-Dip Transformation of Flax Linum usitatissimum to Generate Transgenic Progenies with a High Transformation Rate
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High efficiency Agrobacterium-mediated gene transfer to flax.

L Mlynárová1, M Bauer, J P Nap

  • 1Institute of Plant Genetics, Slovak Academy of Sciences, P.O. Box 39a, 95007, Nitra, Slovak Republic.

Plant Cell Reports
|November 7, 2013
PubMed
Summary
This summary is machine-generated.

Researchers successfully transferred the β-glucuronidase gene into flax plants using Agrobacterium tumefaciens. This study achieved the highest transformation efficiencies reported for flax, generating numerous transgenic shoots efficiently.

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Area of Science:

  • Plant Biotechnology
  • Molecular Biology
  • Genetics

Background:

  • Flax (Linum usitatissimum L.) is an important crop with limited established methods for genetic transformation.
  • Efficient genetic modification is crucial for crop improvement and functional genomics studies in flax.

Purpose of the Study:

  • To establish an efficient Agrobacterium-mediated transformation protocol for flax (Linum usitatissimum L.).
  • To demonstrate the successful transfer and expression of the β-glucuronidase reporter gene in flax.

Main Methods:

  • Agrobacterium tumefaciens-mediated transformation using binary vectors (pAL4404, pBI131).
  • Inoculation and regeneration of flax hypocotyl segments.
  • Selection of transformed tissues using kanamycin resistance.
  • Isolation and subculturing of callus to induce shoot regeneration.

Main Results:

  • Successful integration and expression of the β-glucuronidase gene in flax cultivar Glenelg.
  • Regeneration of numerous transgenic shoots from hypocotyl-derived callus.
  • Achieved the highest transformation efficiencies reported to date for flax.

Conclusions:

  • The developed Agrobacterium-mediated transformation protocol is highly efficient for flax.
  • This method provides a robust platform for generating transgenic flax lines for research and breeding.