Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transgenic Plants02:50

Transgenic Plants

7.0K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.0K
Transgenic Organisms00:53

Transgenic Organisms

29.6K
Overview
29.6K
Transgenic Organisms00:53

Transgenic Organisms

5.4K
5.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Has denigration of career choice within medicine improved in the UK over the last decade?

Education for primary care : an official publication of the Association of Course Organisers, National Association of GP Tutors, World Organisation of Family Doctors·2026
Same author

Evidence of Coherent Elastic Neutrino-Nucleus Scattering with COHERENT's Germanium Array.

Physical review letters·2025
Same author

Intra-temporal and intracranial radiological abnormalities in cochlear implantation.

Irish medical journal·2025
Same author

Contemporary Management of Eustachian Tube Dysfunction.

Irish medical journal·2024
Same author

The TOHU framework: Sensing and interpreting tohu to heal from trauma.

Explore (New York, N.Y.)·2024
Same author

Craniofacial development.

British dental journal·2023
Same journal

The transcriptional dynamics of TIFY/JAZ and BBX families in jasmonate signaling reveal SlBBX17 as a positive regulator of tomato defense.

Plant cell reports·2026
Same journal

CRISPR/Cas9-mediated DFR disruption suggests coordinated changes in flavonoid flux and development in Petunia × hybrida.

Plant cell reports·2026
Same journal

Development and application of KASP molecular marker for accelerated breeding of low-acid Cerasus humilis cultivars.

Plant cell reports·2026
Same journal

Identification of the R2R3-MYB transcription factor family in goji and LbaR2R3-MYB3 mediated wax biosynthesis enhancing powdery mildew resistance in tobacco.

Plant cell reports·2026
Same journal

Diverse molecular mechanisms of in vivo haploid induction in plants: from fertilization interference to genome programming.

Plant cell reports·2026
Same journal

PCIS1 is an essential factor in mitochondrial RNA splicing and complex I biogenesis, with distinct effects in null and downregulated mutants.

Plant cell reports·2026
See all related articles

Related Experiment Video

Updated: May 6, 2026

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector
12:08

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Published on: March 28, 2018

12.2K

Gene insertion into Hevea brasiliensis.

P Arokiaraj1, H Jones, K F Cheong

  • 1Division of Life Sciences, Kings College London, Campden Hill Road, W8 7AH, London, UK.

Plant Cell Reports
|November 7, 2013
PubMed
Summary
This summary is machine-generated.

Scientists developed a particle gun method to genetically modify Hevea brasiliensis. This system successfully introduced foreign genes, like the ß-glucuronidase (gus) gene, into rubber tree cells, paving the way for improved crop development.

More Related Videos

Expression of Fluorescent Proteins in Branchiostoma lanceolatum by mRNA Injection into Unfertilized Oocytes
09:31

Expression of Fluorescent Proteins in Branchiostoma lanceolatum by mRNA Injection into Unfertilized Oocytes

Published on: January 12, 2015

10.5K
Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
09:31

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Published on: April 19, 2019

9.1K

Related Experiment Videos

Last Updated: May 6, 2026

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector
12:08

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Published on: March 28, 2018

12.2K
Expression of Fluorescent Proteins in Branchiostoma lanceolatum by mRNA Injection into Unfertilized Oocytes
09:31

Expression of Fluorescent Proteins in Branchiostoma lanceolatum by mRNA Injection into Unfertilized Oocytes

Published on: January 12, 2015

10.5K
Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
09:31

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Published on: April 19, 2019

9.1K

Area of Science:

  • Plant Biotechnology
  • Molecular Biology
  • Genetics

Background:

  • Hevea brasiliensis (rubber tree) is a vital economic crop.
  • Efficient genetic transformation methods are crucial for improving rubber tree traits.

Purpose of the Study:

  • To establish a reliable particle gun transformation system for Hevea brasiliensis.
  • To demonstrate the successful introduction and expression of foreign genes in rubber tree cells.

Main Methods:

  • Anther-derived calluses of Hevea brasiliensis were transformed using the particle gun method.
  • Vectors contained reporter genes: ß-glucuronidase (gus), neomycin phosphotransferase (nptII), and chloramphenicol acetyl transferase (cat).
  • Gene expression was validated through histochemical staining, fluorometric assays, ELISA, direct enzyme assays, and polymerase chain reaction (PCR).

Main Results:

  • Successful transformation and expression of gus, nptII, and cat genes were confirmed in Hevea callus and embryoids.
  • Significant increases in gene product levels and enzyme activity were observed compared to controls.
  • PCR analysis confirmed the presence of the introduced gus gene fragment.

Conclusions:

  • The particle gun method is a feasible and effective approach for introducing foreign genes into Hevea brasiliensis.
  • This study provides a foundation for genetic engineering of rubber trees for enhanced agricultural applications.