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Characterization of digestive enzyme activity during larval development of gilthead seabream (Sparus aurata)
F J Moyano1, M Díaz, F J Alarcón
1Departmento de Biología Aplicada, Escuela Politécnica Superior, Universidad de Almería, 04120, Almería, Spain.
Abstract:
The evolution of the digestive enzyme equipment in seabream from hatching to 30 days old larvae was studied; there was a progressive increase in the activity of protease, amylase and acid and alkaline phosphatase from day 15 onwards. The use of specific inhibitors, and SDS-PAGE provided evidence to suggest that most of the proteases belonged to the serine group. A high α-amylase activity was also denoted. Zymograms of larval extracts indicated that exogenous food has more a qualitative than a quantitative role in the secretion of digestive enzymes in this species.
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