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Updated: May 6, 2026
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Measurement of Cellular Chemotaxis with ECIS/Taxis
Published on: April 1, 2012
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Antigen-induced eosinophil chemotactic factor (ECF) release by human leukocytes
B M Czarnetzki1, W König, L M Lichtenstein
1Clinical Immunology Division, The Johns Hopkins University School of Medicine at The Good Samaritan Hospital, 5601 Loch Raven Boulevard, Baltimore, Maryland.
Inflammation
|November 7, 2013
Summary
Researchers identified a complement-independent eosinophil chemotactic factor (ECF) released from human leukocytes. This ECF, likely from basophils, parallels histamine release and may be identical to mast cell ECF.
Area of Science:
- Immunology
- Cell Biology
Background:
- Eosinophils play a role in allergic inflammation.
- Leukocytes, including basophils and mast cells, release mediators upon stimulation.
Purpose of the Study:
- To characterize a complement-independent eosinophil chemotactic factor (ECF) from human leukocytes.
- To investigate the relationship between ECF release, histamine release, and cell origin.
Main Methods:
- Peripheral leukocytes from human volunteers were stimulated with antigen or anti-IgE.
- Dose-response and time-release curves for ECF and histamine were analyzed.
- The effect of histamine on eosinophil chemotaxis was assessed at various concentrations.
Main Results:
- A complement-independent ECF was released from leukocytes after antigen or anti-IgE stimulation.
- ECF and histamine release showed parallel dose-response and time-release patterns.
- Histamine did not affect chemotaxis at physiological concentrations but inhibited it at high concentrations.
- Evidence suggests ECF originates from basophils and is similar to mast cell ECF.
Conclusions:
- Human leukocytes release a significant eosinophil chemotactic factor independent of complement activation.
- Basophils are the likely source of this leukocyte-derived ECF, suggesting a shared mediator with mast cells.
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