Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Complexometric Titration: Ligands00:43

Complexometric Titration: Ligands

2.6K
Different monodentate and polydentate ligands are used as complexing agents in complexometric titration reactions. The formation of complexes by mono- and bidentate ligands involves two or more intermediate steps, limiting their use as complexing agents. In comparison, polydentate ligands can form complexes with metal ions in a single-step process, facilitating sharper end points. This means polydentate ligands, such as amino carboxylic acid derivatives, are most commonly employed in...
2.6K
Metal-Ligand Bonds02:51

Metal-Ligand Bonds

19.3K
The hemoglobin in the blood, the chlorophyll in green plants, vitamin B-12, and the catalyst used in the manufacture of polyethylene all contain coordination compounds. Ions of the metals, especially the transition metals, are likely to form complexes.
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...
19.3K
Complexation Equilibria: The Chelate Effect01:19

Complexation Equilibria: The Chelate Effect

1.7K
In complexation reactions, metal atoms or cations interact with ligands to form donor-acceptor adducts called metal complexes. Ligands that bind through one donor site are monodentate, ligands with two donor sites are bidentate, and those with more than two donor sites are polydentate ligands. For example, ethylene diamine is a bidentate ligand that binds through two nitrogen donor atoms, forming a five-membered ring. EDTA is a polydentate ligand that binds through four oxygen and two nitrogen...
1.7K
Ligand Binding Sites02:40

Ligand Binding Sites

11.9K
Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
11.9K
EDTA: Chemistry and Properties01:22

EDTA: Chemistry and Properties

4.2K
Polydentate ligands are most widely used in complexometric titrations because they form more stable complexes with the metal ions than mono- or bidentate ligands due to the chelate effect. Examples of polydentate ligands are ethylenediaminetetraacetic acid (EDTA), crown ethers, and cryptands. The most important feature of optimal polydentate ligands is the ability to form 1:1 complexes in a single-step process. Amino carboxylic acid derivatives are frequently used as complexing agents. EDTA is...
4.2K
Ligand Binding and Linkage00:49

Ligand Binding and Linkage

4.4K
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
4.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Design Rules for Selective Peptide Amphiphile-Gold Nanoparticle Interactions from Atomistic Simulations.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

tRNA-deacylase-directed discovery of biosynthetic pathways.

Nature chemistry·2026
Same author

Exploring the Modularity of Triphenylphosphine-Containing Polymers as Diverse Transition Metal Catalysts.

ACS macro letters·2026
Same author

Biosynthesis of Minimal C-Phycocyanin Chromophore Assemblies in <i>E. coli</i> Provides a Platform to Dissect Protein-Mediated Tuning of Exciton Transfer.

Journal of the American Chemical Society·2026
Same author

Engineered MS2 Virus Capsids for Cellular Display of Peptide Antigens.

ACS chemical biology·2025
Same author

Site-selective protein editing by backbone extension acyl rearrangements.

Nature chemical biology·2025
Same journal

Carbonylative Aminative Suzuki-Miyaura Coupling: Pd-Catalyzed Synthesis of Amides from Vinyl/Aryl Halides and Boronic Acids.

Journal of the American Chemical Society·2026
Same journal

Divergent Asymmetric Synthesis of Glutinosasins A-E.

Journal of the American Chemical Society·2026
Same journal

Ultrastrong Polyketone Hot-Melt Adhesives Enabled by Ni-Catalyzed Carbonylative Polymerization.

Journal of the American Chemical Society·2026
Same journal

Programmable Anomalous Photovoltaics Enabled by Light-Electric Dual-Field Control.

Journal of the American Chemical Society·2026
Same journal

Biomimetic Redox-Mediated Proton Relay in Nanoreactors for Photocatalysis.

Journal of the American Chemical Society·2026
Same journal

The Sulfur Monoxide-Water Complex.

Journal of the American Chemical Society·2026
See all related articles

Related Experiment Video

Updated: May 6, 2026

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
10:17

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

7.3K

Selective chromium(VI) ligands identified using combinatorial peptoid libraries.

Abigail S Knight1, Effie Y Zhou, Jeffrey G Pelton

  • 1Department of Chemistry, University of California , 724 Latimer Hall, Berkeley, California 94720, United States.

Journal of the American Chemical Society
|November 8, 2013
PubMed
Summary
This summary is machine-generated.

Researchers developed novel peptoid-based ligands to selectively capture toxic hexavalent chromium [Cr(VI)] from water. These ligands offer a cost-effective solution for remediating Cr(VI) contamination in environmental water samples.

More Related Videos

Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay
06:17

Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay

Published on: February 28, 2025

1.3K
Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source
08:35

Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source

Published on: May 29, 2021

7.5K

Related Experiment Videos

Last Updated: May 6, 2026

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
10:17

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

7.3K
Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay
06:17

Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay

Published on: February 28, 2025

1.3K
Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source
08:35

Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source

Published on: May 29, 2021

7.5K

Area of Science:

  • Environmental Chemistry
  • Materials Science
  • Biotechnology

Background:

  • Hexavalent chromium [Cr(VI)] is a pervasive global water contaminant.
  • Current remediation strategies for Cr(VI) lack cost-effectiveness and efficiency.
  • A key challenge is the absence of selective ligands capable of binding Cr(VI) amidst numerous other ions in aqueous solutions.

Purpose of the Study:

  • To design and apply a peptoid-based library of ligand candidates for selective Cr(VI) binding.
  • To identify and characterize peptoid sequences with high affinity and specificity for Cr(VI) at neutral pH.
  • To evaluate the efficacy of these peptoid ligands in remediating Cr(VI)-contaminated environmental water samples.

Main Methods:

  • A library of peptoid sequences was synthesized and screened for Cr(VI) binding.
  • UV-vis spectroscopy was used for affinity titrations of identified sequences.
  • NMR spectroscopy was employed for detailed characterization of Cr(VI)-ligand complexes.
  • Peptoid sequences were immobilized on solid-phase resins for remediation experiments.

Main Results:

  • Eleven peptoid sequences capable of binding Cr(VI) under challenging conditions (neutral pH, excess ions) were identified.
  • Affinity comparisons revealed significant binding capabilities among the selected sequences.
  • Characterization elucidated the coordination interactions and specificity determinants.
  • Synthesized peptoid resins effectively reduced Cr(VI) levels in simulated contaminated water samples to EPA-compliant ranges.

Conclusions:

  • Peptoid-based ligands represent a promising new class of selective chelators for toxic metal ions like Cr(VI).
  • These ligands offer a cost-effective and efficient approach for environmental remediation of chromium contamination.
  • The study highlights the potential of peptoid sequences as versatile components for developing advanced environmental remediation materials.