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A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
Published on: February 20, 2012
Different CMS sources found in Beta vulgaris ssp maritima: mitochondrial variability in wild populations revealed by
P Saumitou-Laprade1, G J Rouwendal, J Cuguen
1Laboratory of Genetics and Evolution of Plant Populations, URA CNRS 1185, Scientific and Technical University of Lille, F-59655, Villeneuve d'Ascq CEDEX, France.
Abstract:
Mitochondrial DNA (mtDNA) variation in natural Beta maritima populations has been characterized by way of Southern blot hybridizations of total DNA using non-radioactive probes and chemiluminescent detection. It was found that the previously described N ("normal") mitochondrial type could be subdivided into three subtypes. A new mitochondrial genotype (type R) was distinguished in addition to the previously described type S. Both are male-sterile cytoplasms and can produce a. segregation of sexual phenotypes in their progenies depending on the nuclear background. The populations contained at least two to four different mitochondrial genotypes.
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