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Characterization ofPseudomonas geomorphus: A novel groundwater bacterium
A Breen1, D A Stahl, B Flesher
1Department of Microbiology, University of Tennessee, 37919, Knoxville, Tennessee, USA.
Microbial Ecology
|November 8, 2013
Summary
Researchers identified a novel bacterium, Pseudomonas geomorphus, from an Oklahoma aquifer. This Gram-negative bacterium can stably maintain introduced plasmid DNA, making it a promising candidate for genetic engineering applications.
Area of Science:
- Microbiology
- Environmental Science
- Genetics
Background:
- A novel Gram-negative, pleomorphic bacterium, strain ABS10, was isolated from a pristine aquifer.
- Initial characterization suggested it might be an Arthrobacter sp. due to morphological changes and Gram-variable staining.
Purpose of the Study:
- To characterize strain ABS10 for its potential in introducing and expressing plasmid DNA.
- To identify and classify the novel bacterium.
Main Methods:
- Fatty acid methyl ester profiling
- DNA-DNA hybridization
- 16S rRNA sequence analysis
- Plasmid DNA introduction using broad host range plasmid RP4
Main Results:
- Strain ABS10 showed high similarity to Pseudomonas putida via fatty acid analysis and 81% DNA-DNA homology.
- 16S rRNA sequencing placed it within the Gamma division of purple photosynthetic bacteria.
- The organism was named Pseudomonas geomorphus, reflecting its subterranean origin and morphological changes.
- Broad host range plasmid RP4 was successfully introduced and stably maintained in P. geomorphus.
Conclusions:
- Pseudomonas geomorphus is a novel species identified from an aquifer.
- The ability of P. geomorphus to stably maintain plasmid DNA makes it a suitable host for genetic manipulation.
- Plasmid RP4 can serve as a vector for introducing catabolic genes into this groundwater isolate.
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