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Updated: May 6, 2026

Scalable Transfection of Maize Mesophyll Protoplasts
Published on: June 23, 2023
Transformation of maize (Zea mays L.) protoplasts and regeneration of haploid transgenic plants
K Sukhapinda1, M E Kozuch, B Rubin-Wilson
1DowElanco Biotechnology Laboratory, 9410 Zionsville Rd., P.O. Box 68955, 46268-1053, Indianapolis, IN, USA.
Abstract:
Transgenic haploid maize (Zea mays L.) plants were obtained from protoplasts isolated from microspore-derived cell suspension cultures. Protoplasts were electroporated in the presence of plasmid DNA containing the gus A and npt II genes encoding ß-glucuronidase (GUS) and neomycin phosphotransferase II (NPT II), respectively. Transformed calli were selected and continuously maintained on kanamycin containing medium. Stable transformation was confirmed by enzyme assays and DNA. analysis. Stably transformed tissue was transferred to regeneration medium and several plants were obtained. Most plants showed NPT II activity, and some also showed GUS activity. Chromosome examinations performed on representative plants showed that they were haploid. As expected, these plants were infertile.
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