Starch synthesis in developing wheat grain : The effect of light on endosperm starch synthesis in vitro and in vivo

The effect of light on the in-vivo rate of starch synthesis in the endosperm of developing wheat (Triticum aestivum cv. Mardler) grain was studied. Individual grains from spikelets grown on the same spike either in darkness or bright light showed no difference in their ability to accumulate radioactivity or to convert this to starch over a 14-h period. Similarly, there was no difference in final grain dry weight between spikes which had been kept in either darkness or normal light from 10 d post anthesis. In contrast, when "half-grains" (grain which had been bisected longitudinally along the crease region) were incubated by being submerged in culture solution (in vitro) the incorporation of [(14)C]sucrose into starch was stimulated by increased irradiance. Further experiments showed that the in-vitro dependence on light could be linked to the availability of oxygen. We suggest that in vitro the diffusion of oxygen into the endosperm cells combined with an increased rate of respiration of the tissue during the incubation causes this limitation. Thus the dependence of starch synthesis on light is an artefact of the in-vitro incubation system. The photosynthetic ability of the green pericarp tissue can be used to prevent the development of anoxia in the endosperm tissue of half-grains incubated in vitro. In conclusion, we propose that starch synthesis in vivo is not dependent on oxygen production by photosynthesis in the green layer of the pericarp.