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Trihybrid Crosses

Trihybrid Crosses

Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...

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RAPD markers for constructing intraspecific tomato genetic maps.

M R Foolad¹, R A Jones, R L Rodriguez

¹Dept. of Vegetable Crops, University of California, 95616-8746, Davis, CA, USA.

Plant Cell Reports

|November 8, 2013

Summary

This summary is machine-generated.

Random Amplified Polymorphic DNA (RAPD) technology offers superior potential for generating genetic markers within cultivated tomatoes compared to isozymes and RFLPs. This advancement facilitates the creation of detailed intraspecific genetic maps for tomato breeding.

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Area of Science:

Plant genetics
Molecular biology
Agricultural science

Background:

Existing molecular genetic maps in tomato (Lycopersicon spp.) rely on isozyme and RFLP polymorphisms, which have limited utility for intraspecific analysis within cultivated varieties.
Current markers lack sufficient polymorphism for detailed intraspecific genetic mapping and manipulation in cultivated tomatoes.

Purpose of the Study:

To evaluate the efficacy of Random Amplified Polymorphic DNA (RAPD) technology in generating polymorphic DNA markers within cultivated tomatoes.
To compare the potential of RAPD markers against isozymes and Restriction Fragment Length Polymorphisms (RFLPs) for intraspecific tomato genetic mapping.

Main Methods:

Examined 16 isozymes, 25 RFLP clones, and 313 random oligonucleotide primers.
Assessed polymorphism detection capabilities between cultivated tomato varieties and between Lycopersicon esculentum and Lycopersicon pennellii genotypes.

Main Results:

None of the isozymes and only 16% of RFLP clones showed polymorphism between cultivated tomato varieties.
Up to 63% of RAPD primers detected polymorphic DNA fragments within cultivated varieties.
All RAPD primers successfully identified polymorphisms between L. esculentum and L. pennellii genotypes.

Conclusions:

RAPD technology demonstrates significantly higher potential for generating polymorphic markers within cultivated tomatoes compared to isozymes and RFLPs.
RAPD markers are suitable for constructing high-resolution intraspecific genetic maps, aiding in genetic analysis and breeding of cultivated tomatoes.