Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

16.3K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
16.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The impact of long haul travel on the sleep of elite athletes.

Neurobiology of sleep and circadian rhythms·2023
Same author

Differentiating acute from chronic insomnia with machine learning from actigraphy time series data.

Frontiers in network physiology·2023
Same author

A machine learning model for multi-night actigraphic detection of chronic insomnia: development and validation of a pre-screening tool.

Royal Society open science·2021
Same author

Chromosomal location of genes controlling seed proteins in species related to wheat.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Inheritance of glutenin protein subunits of wheat.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Anti-sense RNAs of cucumber mosaic virus in transgenic plants assessed for control of the virus.

Plant molecular biology·2013

Related Experiment Video

Updated: May 6, 2026

Floral-Dip Transformation of Flax Linum usitatissimum to Generate Transgenic Progenies with a High Transformation Rate
08:49

Floral-Dip Transformation of Flax Linum usitatissimum to Generate Transgenic Progenies with a High Transformation Rate

Published on: December 19, 2014

29.7K

Developing a transposon tagging system to isolate rust-resistance genes from flax.

J G Ellis1, E J Finnegan, G J Lawrence

  • 1Division of Plant Industry, CSIRO, GPO Box 1600, ACT 2601, Canberra, Australia.

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik
|November 8, 2013
PubMed
Summary

Maize transposable elements Ac and Ds were introduced into flax to tag rust resistance genes. Transposition was low in flax, but one family showed potential for transposon tagging of resistance genes.

More Related Videos

Environmentally Induced Heritable Changes in Flax
08:10

Environmentally Induced Heritable Changes in Flax

Published on: January 26, 2011

11.0K
Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis
13:31

Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis

Published on: October 31, 2014

13.3K

Related Experiment Videos

Last Updated: May 6, 2026

Floral-Dip Transformation of Flax Linum usitatissimum to Generate Transgenic Progenies with a High Transformation Rate
08:49

Floral-Dip Transformation of Flax Linum usitatissimum to Generate Transgenic Progenies with a High Transformation Rate

Published on: December 19, 2014

29.7K
Environmentally Induced Heritable Changes in Flax
08:10

Environmentally Induced Heritable Changes in Flax

Published on: January 26, 2011

11.0K
Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis
13:31

Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis

Published on: October 31, 2014

13.3K

Area of Science:

  • Plant genetics
  • Molecular biology
  • Genomics

Background:

  • Flax rust resistance is controlled by multiple genes.
  • Transposable elements offer a method for gene tagging.

Purpose of the Study:

  • To assess the frequency of maize Ac and Ds transposable elements in flax.
  • To determine the potential for transposon tagging of flax rust resistance genes.

Main Methods:

  • Transformation of flax with Ac and Ds elements.
  • Analysis of transposition frequency in callus and plant tissues.
  • Expression of Ac transposase from a cDNA copy.
  • Segregation analysis of transposed elements in progeny.

Main Results:

  • Transposition frequency in flax was lower than in other hosts.
  • Modifications to the Ac transposase gene increased transposition in callus.
  • Transactivation of Ds excision was achieved.
  • One flax family with multiple Ac copies showed transposition in progeny.
  • An active Ac element was mapped near a rust resistance gene.

Conclusions:

  • Transposon tagging in flax is challenging due to low transposition rates.
  • Specific flax families may be useful for resistance gene tagging programs.
  • Further research is needed to optimize transposon activity in flax.