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Updated: May 6, 2026

Quantifying the Binding Interactions Between CuII and Peptide Residues in the Presence and Absence of Chromophores
Published on: April 5, 2022
Kinetic studies of peptide bond formation. Effect of chloramphenicol
1Instituto de Biología Celular, Madrid, Spain.
Abstract:
There might be an undetermined order in the interaction of the substrates with the ribosomes in the reaction of CACCA-Leu-Ac with puromycin to form Ac-Leu-puromycin and CACCA ('fragment reaction'). Km 0°=6×10(-4) M for the puromycin · ribosome interaction. Chloramphenicol totally blocks the 'fragment reaction' as a consequence of a single interaction with the ribosome of Kd 0°=2.2×10(-6) M. The inhibition by chloramphenicol of the 'fragment reaction' is mixed competitive for puromycin.
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