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Updated: May 6, 2026

In Vitro Reassociation Assay to Measure the Formation of 80S Ribosomal Particles Using Salt-washed Ribosomal Subunits
Published on: December 16, 2025
Structural heterogeneity of 30S and 50S ribosomal subunits in Bacillus licheniformis
1Biochemisch Laboratorium, Vrije Universiteit, De Boelelaan 1085, Amsterdam-Buitenveldert, The Netherlands.
Abstract:
Proteins of free and polysome-derived ribosomal subunits of Bacillus licheniformis were fractionated by means of two-dimensional polyacrylamide gel electrophoresis. The free and derived 30S subunits have twelve protein components in common. Five proteins are present on the free 30S subunits only, whereas four proteins are exclusively found on the derived 30S subunits. The free and derived 50S subunits have at least twenty-eight proteins in common. Four proteins are unique for the derived and one for the free 50S subunits.
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