Related Experiment Video
Updated: May 6, 2026
12:21
MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues
Published on: November 30, 2013
14.2K
A microarray analysis of urinary microRNAs in renal diseases
Tsuneo Konta1, Kazunobu Ichikawa, Kazuko Suzuki
1Department of Cardiology, Pulmonology and Nephrology, Yamagata University School of Medicine, 2-2-2 Iida-Nishi, Yamagata, 990-9585, Japan, kkonta@med.id.yamagata-u.ac.jp.
Clinical and Experimental Nephrology
|November 8, 2013
Summary
Urinary microRNAs (miRNAs) show altered profiles in kidney disease patients, correlating with disease severity and type. These findings suggest urinary miRNAs are promising biomarkers for evaluating renal diseases.
Area of Science:
- Nephrology
- Molecular Biology
- Biomarker Discovery
Background:
- MicroRNAs (miRNAs) are endogenous small non-coding RNAs implicated in renal disease pathogenesis.
- Urinary miRNAs (UmiRNAs) represent a potential non-invasive tool for assessing kidney health.
Purpose of the Study:
- To investigate the diagnostic potential of UmiRNAs by analyzing their profiles in various renal diseases.
- To determine the correlation between UmiRNA profiles and clinical/histological parameters of renal disease.
Main Methods:
- Urine samples were collected from 5 healthy controls and 71 patients with renal diseases.
- Microarray analysis was employed to profile UmiRNAs and assess their correlation with clinical and histological data.
Main Results:
- UmiRNA concentrations were elevated in renal disease patients compared to controls, correlating with proteinuria and glomerular sclerosis.
- Microarray analysis identified distinct UmiRNA patterns specific to different renal disease types.
- UmiRNA profiles in urine closely mirrored those in renal tissue, indicating a strong correlation.
Conclusions:
- Urinary miRNAs are significantly correlated with renal pathological changes.
- Distinct UmiRNA patterns observed in urine suggest their potential as novel, disease-specific biomarkers for renal diseases.
More Related Videos
Related Concept Videos
01:28Microbiota of the Urogenital Tract
59
The human urogenital system, once thought to be sterile in healthy individuals, is now recognized as a complex microbial habitat. Advancements in molecular sequencing techniques have revealed that even in healthy adults, the kidneys and bladder harbor microbial populations similar to those found in the distal urethra, albeit in much lower abundance. These resident microorganisms, while generally innocuous, can become opportunistic pathogens under conditions that alter the urogenital...
59
01:22MicroRNAs
21.2K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.2K