[Interferonogenicity of the bacterial lysates in human and murine cell cultures]

The antibacterial JRS vaccine was found to be capable of inducing interferon production in human peripheral blood leukocytes and mouse bone marrow cells. The vaccine induced no interferon in L-929 cells or human diploid M-19 cells. Interferon appeared in the culture fluid within 4-6 hours after induction and reached the maximum levels in 18-24 hours. One-hour contact of the vaccine with leukocytes was sufficient for interferon induction. The interferon generated in human blood leukocytes was partially stable at pH 2.2; heating at 56 degrees C for 30 min reduced its activity 4-fold; antiserum to alpha-interferon inhibited it.