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Induction of apoptotic cell death in HL-60 cells by jacaranda seed oil derived fatty acids

Masao Yamasaki1, Chihiro Motonaga, Marino Yokoyama

  • 1Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki.

Journal of Oleo Science
|November 9, 2013
PubMed

Insights

Jacaranda seed oil, rich in jacaric acid (JA), effectively induces apoptosis in human leukemia HL-60 cells. This anticancer effect is mediated by oxidative stress and can be neutralized by alpha-tocopherol.

Area of Science:

  • Biochemistry
  • Pharmacology
  • Oncology

Background:

  • Fatty acids with conjugated double bonds exhibit potent anticancer properties.
  • Jacaranda seed oil is a potential source of cytotoxic compounds.
  • Understanding the mechanisms of action of natural compounds is crucial for drug discovery.

Purpose of the Study:

  • To investigate the anticancer effects of jacaranda seed oil and its primary component, jacaric acid (JA).
  • To determine the mechanism of cell death induced by JA and JFA in human leukemia HL-60 cells.
  • To assess the role of oxidative stress in the cytotoxic activity of JA and JFA.

Main Methods:

  • Extraction and analysis of fatty acids from jacaranda seed oil.
  • Cytotoxicity assays on human leukemia HL-60 cells treated with JFA and JA.
  • Flow cytometry to analyze cell cycle distribution and apoptosis.
  • DNA fragmentation assays to confirm apoptotic cell death.
  • Assessment of the effect of alpha-tocopherol on JFA- and JA-induced cytotoxicity.

Main Results:

  • Jacaranda seed oil contained 30.9% jacaric acid (cis-8, trans-10, cis-12 octadecatrienoic acid).
  • Fatty acid from jacaranda seed oil (JFA) and JA induced significant cell death in HL-60 cells.
  • Apoptotic cell death was confirmed by increased sub-G1 population and DNA fragmentation.
  • The cytotoxic effects of JA and JFA were abrogated by alpha-tocopherol, suggesting a role for oxidative stress.

Conclusions:

  • Jacaranda seed oil and jacaric acid possess potent apoptotic activity against human leukemia HL-60 cells.
  • The observed cytotoxicity is mediated through the induction of oxidative stress.
  • Alpha-tocopherol can inhibit the anticancer effects of jacaranda seed oil components, highlighting the involvement of oxidative mechanisms.

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