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Trihybrid Crosses

Trihybrid Crosses

Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...

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RFLP analysis of rice (Oryza sativa L.) introgression lines.

K K Jena¹, G Kochert, G S Khush

¹Department of Botany, University of Georgia, 30602, Athens, GA, USA.

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik

|November 9, 2013

Summary

Researchers analyzed rice introgression lines to identify chromosome segments from wild rice. Small segments from O. officinalis were found on most chromosomes, potentially involving novel recombination mechanisms for crop improvement.

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Area of Science:

Plant genetics and breeding
Molecular markers
Crop improvement

Background:

Introgression lines are valuable tools for transferring desirable traits from wild relatives into cultivated crops.
Understanding the extent and nature of introgression is crucial for effective breeding programs.
Rice (Oryza sativa) is a staple food crop, and its improvement through introgression is of significant interest.

Purpose of the Study:

To analyze the introgression of chromosome segments from wild rice (O. officinalis) into cultivated rice (O. sativa).
To identify the location and size of introgressed segments using RFLP markers.
To investigate the potential association of introgressed segments with brown planthopper (BPH) resistance.

Main Methods:

Generation of 52 introgression lines (BC2F8) from crosses between O. sativa and O. officinalis.
DNA analysis using 177 RFLP markers distributed across rice chromosomes.
Identification of informative markers to detect O. officinalis segments.

Main Results:

Twenty-eight RFLP markers identified putative O. officinalis introgressed segments in the introgression lines.
Introgressed segments were detected on 11 out of 12 rice chromosomes, replacing O. sativa alleles.
Segments were small, consistent across generations, and their association with BPH resistance was not unambiguous.

Conclusions:

Small O. officinalis chromosome segments can be successfully introgressed into O. sativa, possibly via nonconventional recombination.
The introgressed segments are distributed across most rice chromosomes.
RFLP markers did not conclusively link specific introgressed segments to brown planthopper resistance in this study.