Reversible changes in rat spermatogenesis induced by an antifertility substance (Gossypol). A histochemical report

Male rats received Gossypol 30 mg/kg body mass daily through intubation for 30 and 60 d. The testes were processed for histological and histochemical lipid staining, thiamine pyrophosphatase (TPPase), and alpha-glycerophosphate dehydrogenase (alpha-GPDH)--examinations. Gossypol induced effects were: various types of deformations of elongated spermatids, and their loss of orientation towards the lamina propria during the maturation phase; displacement of germ cells and occasionally also of Sertoli cells towards the lumen of the seminiferous tubule in form of a "puff". TPPase was observed in the supranuclear region of dislocated round spermatids and pachytene spermatocytes. alpha-GPDH was observed in the split pieces of mid- and tail pieces of elongated spermatids enclosed in the "puff". The induced effects were reversible.