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Updated: May 6, 2026

Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats
Published on: December 7, 2021
Microbial communities in the saturated groundwater environment I: Methods of isolation and characterization of
J Kölbel-Boelke1, B Tienken, A Nehrkorn
1FB 2, Mikrobiologie, Universität Bremen, Leobener Str., D-2800, Bremen 33, FRG.
Abstract:
In this paper we present a method of isolation and morphological and physiological characterization of groundwater bacteria based on numerical taxonomy and cluster analysis, and using a miniaturized test system (microtiter plates). Bacteria were isolated randomly on P-agar, and each strain was characterized in regard to 155 features. The media for biochemical differentiation are listed as well as methods of morphological discrimination. 246 strains of heterotrophic and oligotrophic bacteria, isolated from five water samples from different depths of the saturated groundwater area, were used for optimizing media and test reactions.
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