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Updated: May 6, 2026

Generation of Composite Plants in Medicago truncatula used for Nodulation Assays
Published on: March 27, 2011
Transgenic plants of ramie (Boehmeria nivea Gaud.) obtained by Agrobacterium mediated transformation
D M Dusi1, M Dubald, E R de Almeida
1EMBRAPA/CENARGEN, SAIN Parque Rural, C.P. 02372, 70849-970, Brasília DF, Brasil.
Abstract:
A regeneration and transformation protocol for ramie (Boehmeria nivea Gaud.) is presented. Regeneration was obtained from leaf discs placed on solid B-5 medium (Gamborg et al. 1968) containing adequate concentrations of auxin and cytokinin. Co-cultivation of leaf discs with Agrobacterium tumefaciens and subsequent regeneration resulted in transgenic plants as shown by Southern blot and analysis of expression of the GUS-marker gene.
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