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Rhythmic coronary arterial contractions: changes with time and membrane potential.

K D Keef, G Ross

    The American Journal of Physiology
    |March 1, 1986
    PubMed
    Summary

    Cold storage of rabbit coronary arteries alters their contractile responses and membrane potential. These changes are linked to sodium pump activity and suggest distinct mechanisms regulate early and late reactivity.

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    Area of Science:

    • Cardiovascular Physiology
    • Vascular Smooth Muscle Biology
    • Ionic Transport Mechanisms

    Background:

    • Isolated blood vessels are crucial models for studying vascular function.
    • Cold storage is a common preservation method, but its effects on vascular reactivity require detailed investigation.
    • Understanding the electrophysiological basis of vascular tone is essential for managing cardiovascular diseases.

    Purpose of the Study:

    • To investigate the impact of cold storage on the contractile responses of isolated rabbit coronary arteries.
    • To correlate changes in vascular contractility with alterations in resting membrane potential (Em).
    • To elucidate the role of the sodium pump in mediating these observed changes.

    Main Methods:

    • Isolated rabbit coronary artery segments were subjected to cold storage (2°C for 24 hours).

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  • Contractile responses to potassium chloride (KCl) and 2-(2-aminoethyl)-pyridine (AEP) were measured.
  • Resting membrane potential (Em) was recorded at various time points post-storage.
  • Experiments were conducted at physiological (37°C) and room (22-23°C) temperatures.

    • Main Results:

    • Cold-stored vessels exhibited spontaneous rhythmic contractions upon rewarming.
    • Contractile responses to KCl and AEP showed an initial decline followed by a peak and subsequent decrease.
    • Resting membrane potential (Em) fluctuated, becoming less negative initially and then more negative before returning towards baseline.
    • Non-cold-stored vessels did not display these early contractile or electrophysiological changes.
    • Testing at a lower temperature (22-23°C) prolonged the observed temporal patterns.

    Conclusions:

    • Early contractile responses in cold-stored coronary arteries are modulated by dynamic changes in Em, likely driven by alterations in sodium pump activity.
    • The observed pattern suggests an initial increase followed by a decrease in sodium pump activity.
    • Later changes in vascular reactivity appear to be regulated by a separate, distinct mechanism independent of the initial electrophysiological events.