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Dihybrid Crosses

Dihybrid Crosses

Trihybrid Crosses

Trihybrid Crosses

Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...

Pedigree Analysis

Pedigree Analysis

Pedigree Analysis

Pedigree Analysis

Law of Segregation

Law of Segregation

When crossing pea plants, Mendel noticed that one of the parental traits would sometimes disappear in the first generation of offspring, called the F1 generation, and could reappear in the next generation (F2). He concluded that one of the traits must be dominant over the other, thereby causing masking of one trait in the F1 generation. When he crossed the F1 plants, he found that 75% of the offspring in the F2 generation had the dominant phenotype, while 25% had the recessive phenotype.

Punnett Squares

Punnett Squares

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Related Experiment Video

Updated: May 6, 2026

An Improved Method of RNA Isolation from Loblolly Pine P. taeda L. and Other Conifer Species

An Improved Method of RNA Isolation from Loblolly Pine P. taeda L. and Other Conifer Species

Published on: February 22, 2010

Inheritance of RFLP loci in a loblolly pine three-generation pedigree.

M E Devey¹, K D Jermstad, C G Tauer

¹Institute of Forest Genetics, Pacific Southwest Research Station, USDA Forest Service, 2480 Carson Rd., 95667, Placerville, CA, USA.

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik

|November 9, 2013

Summary

This summary is machine-generated.

Researchers are building a high-density genetic map for loblolly pine (Pinus taeda L.) using restriction fragment length polymorphisms (RFLPs). This map will aid in assessing genetic diversity and improving tree breeding programs.

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Last Updated: May 6, 2026

An Improved Method of RNA Isolation from Loblolly Pine P. taeda L. and Other Conifer Species

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Processing the Loblolly Pine PtGen2 cDNA Microarray

Processing the Loblolly Pine PtGen2 cDNA Microarray

Published on: March 20, 2009

Area of Science:

Plant genetics
Forestry science
Molecular biology

Background:

Developing high-density genetic maps is crucial for understanding complex traits in forest trees.
Loblolly pine (Pinus taeda L.) is a commercially important species, necessitating advanced genetic tools for its improvement.

Purpose of the Study:

To construct a high-density restriction fragment length polymorphism (RFLP) linkage map for loblolly pine.
To identify and characterize polymorphic markers for genetic analysis in this species.

Main Methods:

Utilized loblolly pine cDNA and genomic DNA clones as probes for hybridization.
Screened approximately 200 probes against genomic DNA from a three-generation outbred pedigree.
Employed four restriction enzymes (BamHI, DraI, EcoRI, HindIII) to detect polymorphisms.

Main Results:

Over 56% of single- or low-copy probes detected polymorphisms between parent trees.
Ten probes were successfully used to analyze progeny, revealing Mendelian inheritance of codominant alleles.
Observed segregation of three alleles at one locus and multiple independently segregating loci with some probes.

Conclusions:

Restriction fragment length polymorphisms (RFLPs) are effective for genetic mapping in loblolly pine.
The developed RFLPs can be immediately applied to assess conifer genetic diversity and genotype fingerprinting for tree improvement.