Identification and mapping of the Gli-R3 locus on chromosome 1R of rye (Secale cereale L.)

The progenies of two different rye test-crosses were analyzed for secalin proteins by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using unreduced and reduced aqueous ethanol extracts. Segregation for two high-molecular-weight secalin bands (Glu-R1 or Sec3), one ω-secalin band (Gli-R1 or Sec-1), two 40K γ-secalin bands (Gli-R1 or Sec1) and two ω-type secalin bands (new locus) were studied. One recombinant between ω- and γ-secalins was found in one test-cross. The new locus, designated Gli-R3 or Sec-4, was mapped between Glu-R1 and Gli-R1, more displaced towards Gli-R1. In test-cross 1 recombination between Glu-R1 and Gli-R3 was 33.80±3.22%, and between Gli-R3 and Gli-R1, 12.04±2.21%. In the other test-cross the map distances were relatively similar but smaller, likely due to less recombination within two different species of Secale. Genes coding for 40K γ-secalins at Gli-R1 were likely proximal to the centromere with respect to genes coding for ω-secalins at the same complex locus.