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Agrobacterium-mediated transformation of Citrus stem segments and regeneration of transgenic plants
G A Moore1, C C Jacono, J L Neidigh
1Fruit Crops Department, Institute of Food and Agricultural Sciences, University of Florida, 32611, Gainesville, FL, USA.
Abstract:
A method for Agrobacterium-mediated transformation of Citrus and organogenic regeneration of transgenic plants is reported. Internodal stem segments were co-cultured with Agrobacterium harboring binary vectors that contained the genes for the scorable marker ß-glucuronidase (GUS) and the selectable marker NPT-II. A low but significant percentage (≤ 5%) of the shoots regenerated in the presence of 100 μg/ml kanamycin were GUS(+). Polymerase chain reaction (PCR) analysis confirmed that GUS(+) shoots contained T-DNA. Two plants established in soil were shown to be transgenic by Southern analysis.
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