Related Experiment Video
Updated: May 6, 2026

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
Published on: February 14, 2020
Immature maize spikelets develop and produce pollen in culture
1Department of Biology, University of North Carolina, 27599-3280, Chapel Hill, NC, USA.
Abstract:
Immature maize spikelets have been successfully grown in vitro. Culture conditions were refined to maximize development of normal pollen grains. Kinetin was not required for normal development, in contrast to the absolute requirement for this plant growth regulator for in vitro tassel development. Development occured in all stages sampled, from premeiosis to postvacuolation, and there was no lag in progression through the various stages of development as compared to greenhouse-grown material. Cultured spikelets produced pollen that appeared morphologically normal, accumulated starch and had the normal two sperm nuclei and single vegetative nucleus.
More Related Videos
05:38Using Ustilago maydis as a Trojan Horse for In Situ Delivery of Maize Proteins
Published on: February 8, 2019
09:21Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.
Published on: February 15, 2019
Related Concept Videos
Seed Structure and Early Development of the Sporophyte
Pollination and Flower Structure
Plant Tissue Culture