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Characterization of species-specific repeated DNA sequences from B. nigra
V Gupta1, G Lakshmisita, M S Shaila
1Tata Energy Research Institute, 90 Jorbagh, 110003, New Delhi, India.
Abstract:
The construction and characterization of two genome-specific recombinant DNA clones from B. nigra are described. Southern analysis showed that the two clones belong to a dispersed repeat family. They differ from each other in their length, distribution and sequence, though the average GC content is nearly the same (45%). These B genome-specific repeats have been used to analyse the phylogenetic relationships between cultivated and wild species of the family Brassicaceae.
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