Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Metal-Ligand Bonds02:51

Metal-Ligand Bonds

19.3K
The hemoglobin in the blood, the chlorophyll in green plants, vitamin B-12, and the catalyst used in the manufacture of polyethylene all contain coordination compounds. Ions of the metals, especially the transition metals, are likely to form complexes.
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...
19.3K
Metallic Solids02:37

Metallic Solids

16.4K
Metallic solids such as crystals of copper, aluminum, and iron are formed by metal atoms. The structure of metallic crystals is often described as a uniform distribution of atomic nuclei within a “sea” of delocalized electrons. The atoms within such a metallic solid are held together by a unique force known as metallic bonding that gives rise to many useful and varied bulk properties.
All metallic solids exhibit high thermal and electrical conductivity, metallic luster, and...
16.4K
Composite Masonry Walls01:18

Composite Masonry Walls

2.3K
Composite masonry walls combine multiple wythes of the same or different masonry materials to create a unified structure. These walls feature wythes that are bonded together either through mortar-filled collar joints, grouted spaces, or more commonly, with rigid metal ties and reinforcements, with the use of masonry header units being rare. Metal ties are preferred because they effectively minimize water penetration, as these walls primarily absorb moisture and then release it into the...
2.3K
Metal-Semiconductor Junctions01:24

Metal-Semiconductor Junctions

1.4K
The contact of metal and semiconductor can lead to the formation of a junction with either Schottky or Ohmic behavior.
Schottky Barriers
Schottky barriers arise when a metal with a work function (Φm) contacts a semiconductor with a different work function (Φs). Initially, electrons transfer until the Fermi levels of the metal and semiconductor align at equilibrium. For instance, if Φm > Φs, the semiconductor Fermi level is higher than the metal's before contact. The...
1.4K
Ferrocement01:30

Ferrocement

1.1K
Ferro-cement is a distinctive construction material that represents an innovative variant of reinforced concrete, characterized by its unique composition and the method by which it is formed. Unlike standard reinforced concrete, which relies on larger steel bars for reinforcement, ferro-cement utilizes densely packed layers of mesh or fine rods, fully encased in cement mortar. This composition allows for the creation of structures that are significantly thinner and more flexible than their...
1.1K
Design Example: Dimensioning of Concrete Masonry Construction01:13

Design Example: Dimensioning of Concrete Masonry Construction

495
For the construction of a storeroom using concrete masonry units, it's essential to align the dimensions of the structure with the actual sizes of the blocks and the intended mortar joints. On the site in question, there's a stockpile of concrete masonry blocks with a nominal size of eight by eight by sixteen inches, which are to be used in the construction of the storeroom.
The site engineer has laid out a plan for the storeroom with external dimensions of twelve feet in length and...
495

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Decoding Enzyme-Inhibitor Kinetic Mechanisms by Isothermal Titration Calorimetry: The Case of SARS-CoV-2 3CL<sup>pro</sup>.

Analytical chemistry·2026
Same author

Assessing Metal Ion Assignment Accuracy in Protein Data Bank Models via Elemental Spectroscopy.

Journal of chemical information and modeling·2026
Same author

Allosteric Protein Chemical Shift Perturbations are Ubiquitous.

bioRxiv : the preprint server for biology·2026
Same author

The NMR Exchange Format (NEF): Specification and Applications.

bioRxiv : the preprint server for biology·2026
Same author

Hidden structural states of proteins revealed by conformer selection.

Nature communications·2026
Same author

Loop Plasticity Drives Paralog-Specific Recognition in BET ET Domains.

Journal of chemical information and modeling·2026

Related Experiment Video

Updated: May 6, 2026

Indirect Fabrication of Lattice Metals with Thin Sections Using Centrifugal Casting
08:32

Indirect Fabrication of Lattice Metals with Thin Sections Using Centrifugal Casting

Published on: May 14, 2016

12.0K

DisMeta: a meta server for construct design and optimization.

Yuanpeng Janet Huang1, Thomas B Acton, Gaetano T Montelione

  • 1Center for Advanced Biotechnology and Medicine, Northeast Structural Genomics Consortium, Rutgers University, Piscataway, NJ, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 9, 2013
PubMed
Summary
This summary is machine-generated.

Intrinsically disordered protein regions are crucial for biological processes but hinder structural studies. The DisMeta Server aids researchers by predicting these regions, improving protein sample preparation for NMR and crystallization.

More Related Videos

Fabrication of Mechanically Tunable and Bioactive Metal Scaffolds for Biomedical Applications
09:56

Fabrication of Mechanically Tunable and Bioactive Metal Scaffolds for Biomedical Applications

Published on: December 8, 2015

10.3K
Characterizing Dissipative Elastic Metamaterials Produced by Additive Manufacturing
09:39

Characterizing Dissipative Elastic Metamaterials Produced by Additive Manufacturing

Published on: June 28, 2024

1.9K

Related Experiment Videos

Last Updated: May 6, 2026

Indirect Fabrication of Lattice Metals with Thin Sections Using Centrifugal Casting
08:32

Indirect Fabrication of Lattice Metals with Thin Sections Using Centrifugal Casting

Published on: May 14, 2016

12.0K
Fabrication of Mechanically Tunable and Bioactive Metal Scaffolds for Biomedical Applications
09:56

Fabrication of Mechanically Tunable and Bioactive Metal Scaffolds for Biomedical Applications

Published on: December 8, 2015

10.3K
Characterizing Dissipative Elastic Metamaterials Produced by Additive Manufacturing
09:39

Characterizing Dissipative Elastic Metamaterials Produced by Additive Manufacturing

Published on: June 28, 2024

1.9K

Area of Science:

  • Biochemistry
  • Structural Biology
  • Bioinformatics

Background:

  • Intrinsically disordered regions (IDRs) are prevalent in proteins and vital for cellular regulation and signaling.
  • IDRs present significant challenges for experimental techniques like X-ray crystallography and Nuclear Magnetic Resonance (NMR) spectroscopy.
  • Accurate identification of IDRs is essential for optimizing protein structure determination.

Purpose of the Study:

  • To introduce and evaluate the DisMeta Server as a computational tool for identifying intrinsically disordered regions in proteins.
  • To demonstrate the utility of DisMeta in the rational design of protein constructs for structural studies.
  • To improve the efficiency of protein sample preparation for crystallization and NMR analysis.

Main Methods:

  • DisMeta integrates consensus analysis from eight distinct sequence-based disorder prediction algorithms.
  • The server predicts intrinsically disordered regions, secretion signal peptides, transmembrane segments, and low-complexity regions.
  • The DisMeta Server was utilized by the Northeastern Structural Genomics (NESG) consortium for construct design and optimization.

Main Results:

  • DisMeta provides a consensus prediction of disordered regions, aiding in the identification of challenging protein segments.
  • The tool assists in identifying other protein features such as signal peptides and transmembrane domains.
  • Application of DisMeta by NESG led to improved protein expression, solubility, and enhanced quality of NMR spectra and crystallization outcomes.

Conclusions:

  • DisMeta is a valuable meta-server for predicting intrinsically disordered regions and other protein features.
  • Computational prediction of disordered regions facilitates rational protein construct design.
  • The DisMeta Server enhances the structural genomics pipeline by improving sample quality for NMR and crystallization.