Related Experiment Video
Updated: May 6, 2026
06:40
G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
Published on: March 22, 2018
5.0K
High-throughput, cost-effective verification of structural DNA assembly.
Yandi Dharmadi1, Kedar Patel, Elaine Shapland
1Amyris, Inc., 5885 Hollis Street, Suite 100, Emeryville, CA 94608, USA.
Nucleic Acids Research
|November 9, 2013
Summary
This study introduces a new, cost-effective method for verifying DNA assembly. The high-throughput technique uses in silico screening and automated analysis to accurately check large DNA constructs, making synthetic biology more scalable.
Area of Science:
- Synthetic Biology
- Molecular Biology
- Genomics
Background:
- DNA assembly is crucial for synthetic biology applications, from gene synthesis to whole genomes.
- Current verification methods are costly and challenging for large-scale DNA constructs.
Purpose of the Study:
- To develop a comprehensive, high-throughput solution for structural DNA assembly verification.
- To reduce the cost and logistical burden of verifying large DNA constructs.
Main Methods:
- In silico screening of restriction enzymes.
- Rolling circle amplification of plasmid DNA.
- Capillary electrophoresis and automated digest pattern recognition.
Main Results:
- Successfully screened over 31,000 clones of DNA constructs.
- Achieved a low cost of less than $1 per sample.
- Demonstrated a robust and scalable verification methodology.
Conclusions:
- The described method offers a low-cost, high-throughput solution for DNA assembly verification.
- This approach addresses the scalability challenges in synthetic biology.
- Enables efficient and accurate structural verification of large DNA constructs.
Related Concept Videos
01:57Sanger Sequencing
801.1K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
801.1K
03:00Next-generation Sequencing
88.0K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
88.0K
03:36Genome Annotation and Assembly
16.8K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
16.8K