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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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Related Experiment Video

Updated: May 6, 2026

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A pilot study using next-generation sequencing in advanced cancers: feasibility and challenges.

Glen J Weiss1, Winnie S Liang, Michael J Demeure

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Summary

Next-generation sequencing (NGS) can identify multiple tumor vulnerabilities in advanced cancer patients. This approach shows feasibility for improving treatments, despite initial logistical challenges.

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Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
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Area of Science:

  • Genomics and Oncology
  • Translational Cancer Research

Background:

  • Targeted therapies for advanced cancers show limited success, with eventual tumor progression.
  • Identifying multiple tumor vulnerabilities could offer alternative therapeutic strategies.

Purpose of the Study:

  • To explore the feasibility of using next-generation sequencing (NGS) to identify genomic alterations and expression changes in advanced cancer.
  • To assess the potential of NGS-guided treatment for patients with progressing tumors.

Main Methods:

  • Employed whole genome sequencing (WGS) and whole transcriptome sequencing (WTS) on tumor and normal samples from advanced cancer patients.
  • Investigated genomic events and associated gene expression changes.

Main Results:

  • Completed WGS on nine patients and WTS on six patients.
  • One patient receiving NGS-guided treatment showed a temporary PET/CT response and pain reduction.
  • Identified challenges including reporting delays, communication issues, and sample handling for Clinical Laboratory Improvement Amendments (CLIA) validation.

Conclusions:

  • Demonstrated the feasibility of utilizing NGS in advanced cancer patients.
  • Despite initial delays and logistical hurdles, NGS offers a pathway to potentially improve treatments for progressing cancers.