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Ganoderiol A-enriched extract suppresses migration and adhesion of MDA-MB-231 cells by inhibiting FAK-SRC-paxillin

Guo-Sheng Wu1, Yue-Lin Song, Zhi-Qi Yin

  • 1State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.

Plos One
|November 9, 2013
PubMed

Insights

Ganoderma lucidum extract (GAEE) inhibits breast cancer cell adhesion and migration by disrupting key signaling pathways. This traditional medicine shows potential for breast cancer treatment by targeting focal adhesion kinase and Rho GTPases.

Area of Science:

  • Oncology
  • Pharmacology
  • Cell Biology

Background:

  • Cell adhesion, migration, and invasion are crucial for cancer metastasis.
  • Ganoderma lucidum (Lingzhi) is a traditional Chinese medicine with known anti-cancer properties.

Purpose of the Study:

  • To investigate the effects of Ganoderma lucidum extract (GAEE) on highly metastatic breast cancer cells.
  • To elucidate the molecular mechanisms underlying GAEE's anti-metastatic effects.

Main Methods:

  • Treatment of MDA-MB-231 breast cancer cells with GAEE at non-toxic doses.
  • Analysis of cell adhesion, migration, and invasion.
  • Western blotting to assess protein expression and interactions (FAK, SRC, paxillin, RhoA, Rac1, Cdc42, N-WASP).

Main Results:

  • GAEE significantly inhibited cell adhesion and migration, with a slight effect on invasion.
  • GAEE decreased active focal adhesion kinase (FAK) and disrupted FAK-SRC interaction, leading to paxillin deactivation.
  • GAEE downregulated RhoA, Rac1, and Cdc42, impairing cell migration and actin assembly via N-WASP interaction.

Conclusions:

  • Ganoderma lucidum triterpenoids suppress breast cancer cell migration and adhesion through the FAK-SRC-paxillin signaling pathway.
  • GAEE demonstrates potential as a therapeutic agent for breast cancer treatment.

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