Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calcium binding to cardiac sarcolemma.

J R Williamson, M L Woodrow, A Scarpa

    Recent Advances in Studies on Cardiac Structure and Metabolism
    |January 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Streptozotocin plus 5-fluorouracil followed by everolimus or the reverse sequence in patients with advanced pancreatic neuroendocrine tumors (SEQTOR-GETNE phase III study): a randomized clinical trial.

    ESMO open·2025
    Same author

    Retraction Note: Angiogenic and signalling proteins correlate with sensitivity to sequential treatment in renal cell cancer.

    British journal of cancer·2025
    Same author

    Long-acting lipegfilgrastim and antimicrobials as vigorous primary prophylaxis in bendamustine-treated patients with indolent B cell non-Hodgkin lymphoma: a multicentric real-life experience.

    Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer·2025
    Same author

    Artificial intelligence entering the pathology arena in oncology: current applications and future perspectives.

    Annals of oncology : official journal of the European Society for Medical Oncology·2025
    Same author

    Bendamustine supercharge plus brentuximab vedotin as early salvage therapy following failure to obtain complete metabolic remission after two cycles of adriamycin-bleomycin-vinblastine-dacarbazine for classic Hodgkin lymphoma in patients aged ≤ 60 years: Long-term efficacy results of a retrospective multicentre study.

    British journal of haematology·2025
    Same author

    Corrigendum to "Recommendations for the use of next-generation sequencing (NGS) for patients with advanced cancer in 2024: a report from the ESMO Precision Medicine Working Group": [Ann Oncol 35 (2024) 588-606].

    Annals of oncology : official journal of the European Society for Medical Oncology·2025

    Guinea pig heart sarcolemma has two types of calcium-binding sites. Low-affinity calcium binding, likely external, was inhibited by ruthenium red and verapamil.

    Area of Science:

    • Cardiovascular Physiology
    • Membrane Biochemistry
    • Ion Transport

    Background:

    • The sarcolemma, or cell membrane of cardiac muscle cells, plays a crucial role in regulating intracellular calcium levels, essential for heart contraction.
    • Understanding calcium binding sites on the sarcolemma is key to elucidating excitation-contraction coupling mechanisms.
    • Previous research has identified various ion channels and transporters, but a detailed characterization of sarcolemmal calcium binding sites is ongoing.

    Purpose of the Study:

    • To identify and characterize different classes of calcium (Ca2+) binding sites on guinea pig heart sarcolemma.
    • To investigate the affinity and quantity of these Ca2+ binding sites.
    • To determine the location and potential modulators of low-affinity Ca2+ binding sites.

    Main Methods:

    Related Experiment Videos

    • Isolation of sarcolemma preparations from guinea pig hearts.
    • Measurement of Ca2+ binding using varying concentrations to determine binding kinetics (Km').
    • Assessment of the effect of ruthenium red and verapamil on Ca2+ binding.
    • Attempted investigation of H+ and Ca2+ competition.

    Main Results:

    • Two distinct classes of Ca2+ binding sites were identified: high-affinity sites (Km' = 16 muM) and low-affinity sites (Km' = 800 muM).
    • The number of low-affinity sites was approximately 10 times greater than high-affinity sites.
    • Ruthenium red and verapamil significantly inhibited low-affinity Ca2+ binding.
    • These findings suggest the low-affinity sites are located on the external surface of the sarcolemma.
    • Direct competition between protons (H+) and Ca2+ at these sites could not be conclusively demonstrated.

    Conclusions:

    • Guinea pig cardiac sarcolemma possesses both high- and low-affinity Ca2+ binding sites.
    • Low-affinity Ca2+ binding is likely associated with external membrane components and is sensitive to ruthenium red and verapamil.
    • Further studies are needed to fully elucidate the functional role and precise localization of these sarcolemmal Ca2+ binding sites and their interaction with protons.