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In clinical practice, the direct measurement of hepatic blood flow to evaluate liver function presents significant challenges due to the intricate and specialized nature of the necessary techniques. Consequently, healthcare professionals often rely on empirical estimates derived from thorough patient examinations and liver function tests to gauge liver health. Among the tools at their disposal, the Child–Pugh and MELD scoring systems stand out for their ability to categorize and assess...

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Related Experiment Video

Updated: May 6, 2026

Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy

Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy

Published on: February 7, 2015

Hepatic mitochondrial function analysis using needle liver biopsy samples.

Michael J J Chu¹, Anthony R J Phillips, Alexander W G Hosking

¹Department of Surgery, University of Auckland, Auckland, New Zealand.

|November 9, 2013

Summary

This summary is machine-generated.

This study introduces a new method for assessing liver mitochondrial function (MF) using minimal tissue from a Tru-cut biopsy. This technique allows for direct, reliable measurement of hepatic health, making outpatient liver function assessment feasible.

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Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy

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Published on: September 16, 2020

Area of Science:

Hepatology
Mitochondrial Physiology
Surgical Pathology

Background:

Current pre-operative liver function assessment relies on indirect biochemical and histological methods.
Mitochondrial function (MF) analysis offers direct measurement of cellular metabolic health.
Conventional MF analysis requires large tissue samples (>100 mg), limiting its clinical application.

Purpose of the Study:

To develop and validate a minimally invasive method for assessing liver mitochondrial function (MF).
To determine the feasibility of using small tissue samples (<3 mg) obtained via Tru-cut biopsy for MF analysis.
To establish MF as a direct index of hepatic health suitable for percutaneous application.

Main Methods:

Utilized an 18G Bard® Max-core® biopsy instrument for tissue collection.
Evaluated Tru-cut® biopsy sample weight, stability, and reproducibility in rat and human liver tissues.
Measured MF in saponin-permeabilized samples using high-resolution respirometry.

Main Results:

Successfully assessed MF using <3 mg of tissue from Tru-cut® biopsies (average mass 5.16±0.15 mg for human samples).
Demonstrated low coefficients of variation (<10%) for key MF parameters (State-III respiration, ETC capacity, RCR) in replicate measurements.
Consistently detected lower MF in ischemic liver samples compared to normal perfused samples, confirming assay sensitivity.

Conclusions:

Established a reliable method for measuring liver MF using minimal tissue from a single Tru-cut® biopsy.
Successfully demonstrated the detection of MF derangements in a disease state.
This technique enables outpatient assessment of liver MF, offering a novel tool for evaluating hepatic function.