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Isolation of Adipose Tissue Immune Cells
Published on: May 22, 2013
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Adipose Natural Regulatory B Cells Negatively Control Adipose Tissue Inflammation
Satoshi Nishimura1, Ichiro Manabe2, Satoshi Takaki3
1Department of Cardiovascular Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo 113-8655, Japan; Translational Systems Biology and Medicine Initiative, Graduate School of Medicine, The University of Tokyo, Tokyo 113-8655, Japan.
Cell Metabolism
|November 12, 2013
Summary
Regulatory B (Breg) cells in adipose tissue produce interleukin-10 to control inflammation. Their dysfunction in obesity exacerbates adipose inflammation and insulin resistance, highlighting their crucial role in metabolic homeostasis.
Area of Science:
- Immunology
- Metabolic Diseases
- Adipose Tissue Biology
Background:
- Obesity induces adipose tissue inflammation, contributing to metabolic dysfunction.
- Mechanisms regulating adipose inflammation are not fully understood.
- Regulatory B (Breg) cells modulate immune responses and are implicated in autoimmune diseases.
Purpose of the Study:
- To investigate the role of regulatory B (Breg) cells in adipose tissue inflammation and obesity.
- To identify factors supporting Breg cell function in adipose tissue.
Main Methods:
- Analysis of B cell populations and interleukin-10 production in adipose tissue.
- Genetic manipulation of Il10 in B cells in diet-induced obese mice.
- Adoptive transfer of adipose tissue Breg cells.
- Assessment of adipose tissue inflammation and insulin resistance.
- Evaluation of adipose tissue from obese mice and humans.
Main Results:
- Adipose tissue harbors a distinct Breg cell subset constitutively producing interleukin-10.
- B cell-specific Il10 deletion worsened adipose inflammation and insulin resistance in obese mice.
- Adoptive transfer of adipose tissue Breg cells improved metabolic parameters.
- Adipose environmental factors (CXCL12, free fatty acids) support Breg cell function.
- Breg cell numbers and function are reduced in obese mice and humans.
Conclusions:
- Adipose tissue Breg cells are a critical regulatory subset maintaining local immune homeostasis.
- Dysfunction of adipose tissue Breg cells is a key driver of adipose inflammation in obesity.
- Restoring Breg cell function may offer therapeutic strategies for obesity-related metabolic abnormalities.
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