Related Experiment Video

Updated: May 6, 2026

Isolation and Flow Cytometric Assessment of Neuroimmune Interactions in a Mini-Stroke Murine Model
08:22

Isolation and Flow Cytometric Assessment of Neuroimmune Interactions in a Mini-Stroke Murine Model

Published on: June 20, 2025

578

Involvement of Mincle and Syk in the changes to innate immunity after ischemic stroke

Yukiya Suzuki1, Yusuke Nakano, Keisuke Mishiro

  • 11] Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical University, Gifu 501-1196, Japan [2] Laboratory of Drug Informatics, Gifu Pharmaceutical University, Gifu, 501-1196, Japan.

Scientific Reports
|November 12, 2013
PubMed

Insights

Post-ischemic inflammation involves Mincle, a C-type lectin-like receptor, and Syk signaling in ischemic stroke. Inhibiting Syk with piceatannol reduced stroke damage, highlighting Mincle-Syk as a therapeutic target.

Area of Science:

  • Immunology
  • Neuroscience
  • Pathology

Background:

  • Post-ischemic inflammation is crucial in ischemic stroke, with Toll-like receptors (TLRs) implicated.
  • The role of other innate immune receptors, like Mincle (Macrophage-inducible C-type lectin), in cerebral ischemia remains unclear.

Purpose of the Study:

  • To investigate the role of Mincle and its signaling pathway in the pathogenesis of ischemic stroke.
  • To evaluate the therapeutic potential of inhibiting the Mincle-Syk pathway.

Main Methods:

  • Upregulation of Mincle, SAP130, and phospho-Syk/Syk was assessed post-ischemia in mice and human brain samples.
  • Mice were treated with piceatannol, a Syk inhibitor, to evaluate its effects on infarct volume, swelling, and molecular markers.
  • Levels of phospho-Syk, MMP9, ICAM-1, and Claudin5 were analyzed in treated and control groups.

Main Results:

  • Mincle, SAP130, and phospho-Syk/Syk were upregulated following ischemia.
  • Mincle expression was observed in both immune and non-immune cells in ischemic brain tissue.
  • Piceatannol treatment significantly reduced infarct volume and brain swelling.
  • Downregulation of phospho-Syk, MMP9, and ICAM-1, and upregulation of Claudin5 were observed in the piceatannol-treated group.

Conclusions:

  • The Mincle-Syk signaling pathway is activated in ischemic stroke and contributes to pathogenesis.
  • Inhibition of Syk offers a potential therapeutic strategy for mitigating ischemic stroke injury.
  • These findings underscore the critical role of the innate immune system in post-ischemia reperfusion injury.

More Related Videos

Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain12:14

Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain

Published on: February 12, 2016

35.7K
Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain Injury13:28

Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain Injury

Published on: September 4, 2013

11.1K

Related Concept Videos

Ischemic Stroke ll: Pathophysiology01:15

Ischemic Stroke ll: Pathophysiology

An ischemic stroke occurs when a cerebral blood vessel becomes obstructed, most often by a thrombus or embolus, interrupting the delivery of oxygen and glucose to brain tissue. Because neurons rely on continuous aerobic metabolism, energy failure begins within minutes of reduced perfusion. The region receiving the least blood flow becomes the infarct core, an area of irreversible cellular death. Surrounding this core lies the penumbra, a zone of hypoperfused but still viable tissue that is...
68
Ischemic Stroke l: Introduction01:15

Ischemic Stroke l: Introduction

Ischemic stroke is an acute cerebrovascular condition in which blood flow to a brain region is suddenly interrupted, leading to tissue infarction. Neurons depend on continuous oxygen and glucose supply, so even brief reductions in perfusion cause energy failure, ionic imbalance, and irreversible injury. Ischemic strokes are classified into thrombotic and embolic types based on their underlying mechanisms.Thrombotic MechanismsThrombotic stroke develops when a clot forms within a cerebral artery.
53
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
2.8K